EMAIL THIS PAGE TO A FRIEND
Sigma

11-4

11033102

Synonym: 11-4

Purchase

Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   human cervix
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice

Description

Cell Line Origin

HeLa, genetic modification, firefly luciferase reporter gene, HSV-thymidine kinase promoter

Cell Line Description

11-4 is a HeLa cell line stably transfected with a firefly luciferase reporter gene under the control of the HSV-thymidine kinase promoter. 11-4 can be used to evaluate the non-specific transcriptional activity of chemicals.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 9,10
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442) + 16 μg/ml Blasticidin S HCL

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10, i.e. seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr Noriyuki Suzuki, Sumitomo Chemical Co Ltd, 1-98 Kasugadenaka 3-chrome, Konohana-ku, Osaka 554-8558, Japan

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Pipette Controller from Brand
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?