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Sigma

TKE2

from mouse (CD-1), female eye, 11033107

Synonym: TKE-2, TKE

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse (CD-1), female eye
growth mode   Adherent
karyotype   Normal karyotype of the female mouse CD1 strain
morphology   Epithelial; small and regular cobblestone-like shape
products   Stains positive for nuclear p63 expression and K14 keratin.
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice

Description

Cell Line Origin

Mouse cornea epithelial progenitor cells

Cell Line Description

TKE2 is the first cell line of mouse corneal epithelial progenitor cells isolated from the basal layer of the limbal epithelium.TKE2 has applications in the cell biology of corneal epithelial cells, toxicity, and differentiation. TKE2 can be induced to differentiate into stratified epithelium on increasing the calcium concentration and addition of foetal bovine serum.

DNA Profile

Not specified

Culture Medium

Stemline Keratinocyte Medium II (Sigma S0196) + Stemline Keratinocyte Growth Supplement (Sigma S9945) + 2 mM Glutamine.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 2 x 102 - 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C. It is important to inactivate the trypsin with an equal volume of trypsin inhibitor. To remove all traces of trypsin and trypsin inhibitor pellet the cells by centrifugation and resuspend the cells in fresh medium. Media change every second day.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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