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Sigma

YTH 89.1

11060814, from Rat x Rat Hybridoma, Reactivity human glycophorin A (CD235a)

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   Rat x Rat Hybridoma
ImmunoDon   DA rat spleen
Myeloma   Y3.AG.1.2.3
Reactivity   human glycophorin A (CD235a)
application(s)   cell culture | mammalian: suitable
isotype   IgG2b
shipped in   dry ice

Description

Application

Not specified

Immunogen

Human peripheral blood mononuclear cells

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Cambridge Enterprise Limited, Hauser Forum, 3 Charles Babbage Road, Cambridge CB3 0GT. Originators: Professor H Waldmann and Dr SP Cobbold when at the Department of Pathology, University of Cambridge

Cell Line Origin

Monoclonal antibody human CD235a glycophorin A

Culture Medium

Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.

Subculture Routine

Maintain cultures between 3-9 x 105 cells/ml. 5% CO2; 37 °C. Saturation density 9 x 105/ml

Cell Line Description

Rat hybridoma producing antibody against human glycophorin A CD235a. Cambridge Enterprise Reference number AB66:www.enterprise.cam.ac.uk.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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