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Sigma

SDM103T2

from human lung(effusion), 11120712

Synonym: SDM 103T2

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human lung (effusion)
growth mode   Adherent
karyotype   Not specified
morphology   Epitheloid/mesenchymal
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice

Description

Cell Line Origin

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

DNA Profile

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 11
D13S317: 8
D16S539: 11
D5S818: 11,12
D7S820: 9,10
THO1: 8,9.3
TPOX: 8
vWA: 17

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS)

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 1-4x10,000 cells/cm².

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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