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Sigma

ZL5

from human lung(effusion), 11120715

Synonym: ZL 5, ZL-5

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human lung (effusion)
growth mode   Adherent
karyotype   Modal
morphology   Spheroidal
products   Not specified
receptors   Mesothelial-membrane antigen ME1
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

DNA Profile

STR-PCR Data:
Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,13
D16S539: 12
D5S818: 12
D7S820: 10,11
THO1: 6,9.3
TPOX: 8,11
vWA: 16,17

Culture Medium

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 15% Foetal Bovine Serum (FBS)

Subculture Routine

Split subconfluent cultures (70-80%) 1:3 to 1:6 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 2-4x10,000 cells/cm². The cells in suspension should be collected by centrifugation and added to the trypsinized cells before reseeding.

Cell Line Description

ZL5 was derived from the pleural effusion of a 43-year old male patient, prior to treatment, with a known history of exposure to asbestos.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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