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Sigma

SPC212

11120717

Synonym: SPC 212, SPC-212

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human lung
growth mode   Adherent
karyotype   Not specified
morphology   Epitheloid/mesenchymal
receptors   Mesothelial-membrane antigen ME1
relevant disease(s)   cancer

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Emanuela Felley-Bosco, Laboratory for Molecular Oncology, University Hospital Zurich, Haeldeliweg 4, 8044 Zurich, Switzerland.

Cell Line Origin

A human mesothelioma cell line derived from a malignant mesothelioma. Asbestos exposure

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 9
D5S818: 11,12
D7S820: 8,10
THO1: 6,9
TPOX: 8
vWA: 16

Culture Medium

DMEM:F12 (1:1) (D8062) + L-Glutamine (G7513) + 15% FBS / FCS (F2442).

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 1-4 x 10,000 cells/cm2.

Cell Line Description

SPC212 was derived from a biphasic tumour of the pleural cavity of a 47-year old female patient, prior to treatment, with a known history of exposure to asbestos.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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