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Me9-TG3

11120841

Synonym: Me9, TG3

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Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   rat lung
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial and spindle shaped biphasic morphology
receptors   Not specified
relevant disease(s)   metastasis

Description

Cell Line Origin

Rat transplantable mesothelioma, asbestos, primary tumour

Cell Line Description

The Me-series of mesotheliomas were generated by an intrapleural inoculation of UICC crocidolite to induce primary mesotheliomas in syngenic PVG/c Norweigan Hooded rats. Solid fragments of the tumours were transplanted and maintained by s.c. serial passage. The cell lines are metastatic in a syngeneic host.

DNA Profile

Not specified

Culture Medium

DMEM (D6546)+ L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Split subconfluent cultures (70-80%) 1:4 to 1:10 using 0.25% trypsin/EDTA; 5% CO2; 37 °C. Suggested seeding density 2-4 x 10,000 cells/cm2.

Other Notes

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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