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U4C cell line human

12021505

Synonym: 2fTGH-U4C, 2fTGH

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human fibrosarcoma
growth mode   Adherent
morphology   Epithelial
shipped in   dry ice
storage temp.   −196°C

Description

DNA Profile

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 12
D13S317: 12,14
D16S539: 9,12
D5S818: 11,13
D7S820: 9,10
THO1: 6
TPOX: 8
vWA: 14,19

Culture Medium

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-4 x 10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

The human sarcoma cell line HT 1080 (ECACC Catalogue number 85111505) was transfected with a vector encoding a selectable marker (guanine phosphoribosyltransferase) regulated by alpha interferon to create the 2fTGH cell line, enabling the selection of mutations in genes encoding components of the interferon signalling pathway. Chemical mutagenesis of the parent 2fTGH cell line enabled the isolation of a number of IFNg signalling mutants available from ECACC: U2A (12021502), U3A (12021503), U4A (12021504), U4C (12021505), U5A (12021506), U6A (12021507). Each contains a different mutation in the IFN signalling pathway. The 2fTGH panel provides a tool for the in vitro study and comparison of disrupted interferon signalling at multiple points across the IFN pathway. The U4C mutant lacks the JAK1 kinase. U4C is unresponsive to interferon alpha and interferon gamma; furthermore it is partially defective in response to double stranded RNA.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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