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293T cell line human

12022001

Synonym: 293 T, 293T cell line human, 293tsA1609neo

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Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   human kidney (fetal)
growth mode   adherent
morphology   small slightly rounded cells growing in a dispersed manner
products   SV40 T antigen
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Description

The 293T cell line was created in the laboratory of Michele Calos by transfection of a sub-line of adenovirus-immortalized human embryonic kidney cells with a gene encoding the SV40T-antigen and a neomycin resistance gene. The cell line is competent for replication of vectors carrying the SV40 origin of replication. The line also has favourable tissue culture, transfection, DNA replication, gene expression, and protein production properties. It gives high titres when used to produce many viral vectors such as oncoretroviruses and lentiviruses.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

Culture Medium

DMEM + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 seeding at 2-4 x 104 cells/cm2.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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