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LIM2405 cell line human

12062003

Synonym: LIM 2405, LIM-2405

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Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   human colon
growth mode   adherent
morphology   epithelial
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Description

Human adenocarcinoma of the caecum of a male patient. Cell Bank Australia reference number: CBA0165.

Culture Medium

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1μg/ml, 1-Thioglycerol 10 μM

Subculture Routine

Optimal split ratio 1:4-1:6 (seeding density 1.6 x 104 cells/cm2). Cells incubated at 37°C with 5% CO2. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM2405 may take 2-3 days to seed after thawing or trypsinisation.

Other Notes

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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