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Sigma

UKRV-Mel-14b

from human lymph node, 13012529

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human lymph node
description   Human melanoma, metastasis
growth mode   Adherent
morphology   Epithelial
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

DNA Profile

STR-PCR Data:

Amelogenin: X
CSF1PO: 12
D13S317: 10,12
D16S539: 11
D5S818: 11,12
D7S820: 8,10
THO1: 7,9
TPOX: 8,9
vWA: 15,17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) seed new cultures at 1-3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 8 x 10,000 cells/cm² .

Cell Line Description

UKRV-Mel-14b (ESTDAB-225) was established from the lymph node metastasis in a 52 year-old female patient with metastatic melanoma (superficial spreading melanoma stage IV). The UKRV-Mel-14b cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commission’s fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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