from Rat x Rat Hybridoma, 13082227



Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   Rat x Rat Hybridoma
ImmunoDon   Rat DA spleen
Myeloma   Y3
Reactivity   Mouse CD147
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C


Cell Line Description

The hybridoma cell line OX-114 recognises mouse CD147 secretes monoclonal IgG1 antibodies reactive with rat L-selectin.


Recombinant CD147


The isotype of the monoclonal antibody produced by this cell line is rat IgG1.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Subculture Routine

Seed at 5 x 104 cells/ml (5 x 105 cells/ml at resuscitation). Subculture when cells reach 5 x 105 - 1 x 106 cells/ml (usually after 3-4 days), 5% CO2, 37°C. 20% FBS can be used at resuscitation.

Other Notes

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.


Certificate of Analysis (COA)

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