EMAIL THIS PAGE TO A FRIEND
Sigma

ULK1 KO MEF (SV40)

from mouse embryo, 14050807

Purchase

Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse embryo
description   Mouse embryonic fibroblast, autophagy, Unc51-like kinase, SV40-immortalised
growth mode   Adherent
morphology   Fibroblast
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Description

Mouse embryonic fibroblasts (MEFs) derived from wild-type littermate to the ULK1-knockout mouse. MEFs that were isolated and cultured were immortalised by SV40 large T-antigen.

Culture Medium

DMEM + 10% FCS + 2mM Glutamine

Subculture Routine

Trypsinize with 0.05% Trypsin/EDTA and inoculate new cultures at 2 x 104 per cm2. Cultures must be incubated in a humidified 5% CO2/95% air incubator at 37oC.

Other Notes

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?