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MRC-5 SV2

84100401

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Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   human lung (fetal)
growth mode   Adherent
karyotype   Modal no. = 60-80, hyperdiploid
morphology   Fibroblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human foetal lung, SV40 transformed

Cell Line Description

Derived from the parental cell line MRC-5 by transformation with the SV40 virus. The cells do not senesce.

Application

Transformation and virus studies: SV40

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 11
D5S818: 11,12
D7S820: 10
THO1: 8
TPOX: 8
vWA: 15

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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