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OX-8 Cell Line from murine myeloma

84112009, Reactivity: Anti- CD8, rat

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   Mouse x Mouse Hybridoma
ImmunoDon   BALB/c mouse spleen
Myeloma   NS1
Reactivity   Anti-CD8, rat
application(s)   cell culture | mammalian: suitable
isotype   IgG1
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse NS1 x Mouse BALB/c spleen

Cell Line Description

The antibody recognises subset of rat peripheral T cells that possess suppresser and cytotoxic activities. antibody labels all peripheral T cells that are unlabelled by W3/25. 90% of thymocytes are labelled by both Antibodies. NK cells express the OX-8 antigen.

Immunogen

High molecular weight rat thymocyte glycoproteins

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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