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Sigma

VERO Cell Line

from African green monkey kidney, 84113001, A fibroblast-like kidney cell, non-WHO origin, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Properties

Related Categories CITES, Cell Culture, ECACC Cell Lines, S-Z
biological source   African green monkey kidney
growth mode   Adherent
karyotype   2n = 60, modal no. 58
morphology   Fibroblast-like
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Virus studies: polio, rubella, arboviruses and reoviruses

Cell Line Origin

Monkey African Green kidney

Culture Medium

(D6546) + 2 mM (G7513) + 10% (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Cell Line Description

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

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