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Sigma

P3X63Ag8

85011401

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Properties

Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   mouse skin
growth mode   Suspension
karyotype   Aneuploid
morphology   Lymphoblast
products   Immunoglobulin G (IgG1) (kappa)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse myeloma

Cell Line Description

Derived from the P3X27 cell line, itself a derivative of mineral oil-induced plasmacytoma MOPC-21 in BALB/c mice. Resistant to 20μg/ml azaguanine, does not grow in selective medium. HGPRT neg., dies in HAT. Doubling time 16-26 h.

Application

Fusion partner

Culture Medium

DMEM or RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 5x10,000 to 3x100,000 cells/ml; 5% CO2; 37°C. Cells will achieve maximum cell density of 5x100,000 cells/ml.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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