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B95-8

from cotton-top tamarin monkey blood, 85011419, peripheral blood lymphocyte, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Properties

Related Categories A-C, CITES, Cell Culture, ECACC Cell Lines
biological source   cotton-top tamarin monkey blood
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblast/Fibroblast
products   Epstein Barr Virus (EBV)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Cotton-top Tamarin Monkey peripheral blood lymphocyte

Cell Line Description

Derived from a cotton-top tamarin (Saguinus oedipus). Releases high titres of transforming Epstein Bar virus (EBV). Thus it provides a source of EBV to establish continuous lymphocytic lines from human donors. The cells should be handled under laboratory containment level 2.

In some instances the cell line B95-8 has been described as derived from ′marmoset′; however, this is not correct. B95-8 was derived from a cotton-top tamarin (Saguinas oedipus); this has been confirmed by DNA profiling.

Culture Medium

RPMI 1640 (R0883)+ 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml. May need to use 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. It is recommended that this cell line be frozen at no less than 8x1000,000 cells per ampoule.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
UN3373 - class 6.2 Biological substance, Category B
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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