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Sigma

P3/NS1/1-Ag4.1

85011427

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Properties

Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   mouse blood
growth mode   Suspension
karyotype   Not specified
morphology   Not specified
products   Synthesises kappa-light chain of Immunoglobulin G (IgG1) but does not secrete it
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse myeloma

Cell Line Description

P3/NS1/1-Ag4-1 is a non-Ig-secreting but Kappa light chain synthesising subclone of P3X27. Resistant to 20μg/ml azaguanine and does not grow in selective medium.

Application

Fusion partner

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-8x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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