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Vero C1008 [Vero 76, clone E6, Vero E6]

85020206, from African green monkey kidney, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

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Properties

Related Categories CITES, Cell Culture, ECACC Cell Lines, S-Z
biological source   African green monkey kidney
growth mode   Adherent
karyotype   2n = 60
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Monkey African Green kidney

Cell Line Description

A subclone of Vero76 (Sigma catalog no. 85020205) exhibiting a similar range of virus susceptibility (haemorrhagic viruses). They show some degree of contact inhibition and are suitable for supporting the growth of slowly replicating viruses.

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37 °C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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