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Sigma

WEHI-231

85022107

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse blood
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblast
products   Surface Immunoglobulin M (IgM)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Tumourigenicity studies

Cell Line Origin

Mouse B cell lymphoma

Culture Medium

DMEM + 2mM Glutamine + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:8 to 1:10 i.e. seeding at 2-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

Expresses surface IgM but does not secrete Ig. Secretion of IgM can be induced with LPS. WEHI 231 is a B cell lymphoma of BALB/c x NXB F1 origin induced by mineral oil injection.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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