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Sigma

RAMOS Cell Line human

85030802

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Properties

Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   human lymph
description   Human Caucasian Burkitt′s lymphoma
growth mode   Suspension
karyotype   Modal no. 45
morphology   Lymphoblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Tumourigenicity and virus studies: Epstein-Barr virus (EBV).
RAMOS Cell Line human has been used to carry out a MTT (colorimetric assay) cell viability assay to measure calcium ionophore-induced cell death in Ramos cells under different conditions and to determine the expression of ZFP36 ring finger protein like 1 (ZFP36L1) in Ramos B cells during different stages of B cell differentiation.

Cell Line Origin

Human Caucasian Burkitt′s lymphoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13,14
D16S539: 10,13
D5S818: 7,12
D7S820: 11
THO1: 7,9.3
TPOX: 8,9
vWA: 15,16

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

Derived from a Burkitt′s lymphoma which does not possess the EBV genome. EBV infectability and permanent conversion into EBV positive sub-lines is possible by in vitro infection. The cells have B lymphocyte characteristics, with surface associated mu and kappa chains.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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