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B50

85042302

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Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   rat nerve
growth mode   Adherent
karyotype   Not specified
morphology   Neuronal
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Rat nervous tissue neuronal

Cell Line Description

Ethyl nitrosourea induced tumour cell line. Neuronal cells are useful in the research of the distribution of neurotransmitter synthesis and brain specific antigens among nerve and glia.

Application

Neurobiological studies

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Pipette Controller from Brand
Protocols & Articles
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Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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