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Sigma

U266B1

85051003

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human blood
growth mode   Suspension
karyotype   Not specified
morphology   Lymphoblast
products   IgE
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human myeloma

Cell Line Description

This cell line was derived from peripheral blood of a patient with an IgE myeloma (epsilon2,lambda2). The line has been reported to secrete IgE and is used as fusion partner for the production of hybridomas. U266B1 has been reported to produce interleukin-6 (IL-6).

Application

Fusion partner

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12,13
D13S317: 12
D16S539: 10
D5S818: 11,12
D7S820: 11,12
THO1: 5,7
TPOX: 8
vWA: 17

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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