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Sigma

111A3

85062504, Application: Fusion partner in the production of heterohybridomas

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   Mouse x Bovine Heterohybridoma
ImmunoDon   Jersey calf lymph node
Myeloma   53B3 (mouse x bovine heteromyeloma)
Reactivity   Application: Fusion partner in the production of heterohybridomas
application(s)   cell culture | mammalian: suitable
isotype   Not specified
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse 53B3 x Bovine Jersey Calf lymph node

Cell Line Description

Non Ig secreting

Immunogen

Sheep red blood cells

Application

This line has been used as a HAT sensitive fusion partner in the production of heterohybridomas

Culture Medium

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 10% Horse Serum (HS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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