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Sigma

Y1

85062807

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse adrenal gland (cortex)
growth mode   Adherent
karyotype   2n = 40
morphology   Epithelial
products   Steroid hormone
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse LAF1 adrenal cortex tumour

Cell Line Description

This cell line is a cloned strain from an adrenal cortex tumour cell line of a male LAF1 mouse. This strain was isolated after alternate passage in tumours in vivo and in cultures in vitro . The isolated clones are reported by the original depositor to produce steroid hormones at a high rate (4ug/mg/hr) under ACTH stimulation and cells round up within minutes of ACTH treatment. The hormone produced is reported to be ketosteroid with a hydroxy group at the carbon 20 rather than the usual ketosteroid configuration. However, a laboratory independent to the depositor has since (Dec 2000) tested this steroid producing property using the same method and after 1 hour of stimulation could report no rounding up or change in morphology and a very low cAMP measurement. This laboratory did find that the cells round up when exposed to forskolin (acting through adenylate cyclase) and the original depositor reported morphological changes in response to cholera toxin.

Application

Cloning and hormone studies

Culture Medium

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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