EMAIL THIS PAGE TO A FRIEND
Sigma

A431 Cell Line human

85090402, squamous carcinoma

Purchase

Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   human skin
growth mode   Adherent
karyotype   Hypertriploid
morphology   Epithelial
products   Not specified
receptors   Epidermal growth factor (EGF)
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human squamous carcinoma

Cell Line Description

Derived from an epidermal carcinoma of the vulva taken from an 85 yr old female. The cells carry large numbers of EGF binding sites and is an indicator cell for anti-TGF binding.

Application

A431 Cell Line human has been used:
• to demonstrate that p38 mitogen-activated protein kinase enhances epidermal growth factor-induced apoptosis in A431 cells
• to study melanoma-specific CD44 (a tumor marker and transmembrane receptor) alternative splicing pattern in A431 cells
• to study the anti-cancer activity of antioxidant-rich Cameroonian medicinal plants

Cell cycle studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

???PDP_SDS_LOT_NUMBER_ERROR_MSG???

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
Howdo Ihandle Cell Linefrozen cells upon arrival?
Upon receipt, frozen ampules should be transferred directly to vapor phase liquid nitrogen without delay (-135°C) or liquid phase liquid nitrogen if vapor phase is not available. DO NOT use a -80°C freezer as an alternative; this will result in loss of viability.
Why is Vapor Phase Liquid Nitrogen preferred for storage of Cell Linecells?
If ampules are immersed into liquid phase of liquid nitrogen, it increases the risk of the liquid seeping into the vial. This could lead to problems of cross-contamination and increased risk of the ampule exploding when thawed.
How do I resuscitate/thaw frozen Cell Line cells?
A protocol for thawing cells can be found in the ECACC handbook.
What medium should I use when I thaw the Cell Linecells?
The medium for each cell line is listed on the product page. It is specific to each cell line. If not visible on the Sigma-Aldrich website product page, the HPA website also contains the same information (www.hpacultures.org.uk). The product number for the cell line is the same on either site.
What passage are my Cell Line cells?
If the passage of the cells is known, it is listed on the product page at the HPA website (www.hpacultures.org.uk).
How many Cell Linecells are in the vial?
Each vial of cells contains 2-3 x 106 cells in 1 ml of freezing media. This is in a 1.8 mL ampule. For exceptionally large cells, counts may decrease. Suspension cells, generally smaller cells, may contain as many as 4-5 x 106 cells/vial to assure optimal viability upon thaw.
Are my Cell Linecells mycoplasma tested?
ECACC routinely tests all manufactured cell banks for mycoplasma.
How to freeze down Cell Linecells to make a cell stock?
A protocol for freezing down cells for cell stocks can be found in the ECACC manual.
Are the Cell Linecells tested for viral pathogens?
HPA Cultures does not perform any viral testing on the cell lines.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions

Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?