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85111502

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Properties

Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   human embryo
growth mode   Adherent
karyotype   Modal no. 56
morphology   Spindle shaped
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Caucasian embryo rhabdomyosarcoma

Cell Line Description

Derived from a malignant embryonal rhabdomyosarcoma from a 7 year old Caucasian female. Also designated TE 32 and 130T in the literature

Application

Tumourigenicity studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 13
D16S539: 10,11
D5S818: 11
D7S820: 8,12
THO1: 9.3
TPOX: 9
vWA: 18

Culture Medium

DMEM + 2mM Glutamine + 2% Non Essential Amino Acids (NEAA) + 2% Vitamins + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Other Notes

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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