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Sigma

SK.N.SH Cell Line human

from human nerve(bone marrow metastasis; Caucasian neuroblastoma), 86012802

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human nerve (bone marrow metastasis; Caucasian neuroblastoma)
growth mode   Adherent
karyotype   2n = 46, modal no. 47
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Human SK.N.SH cell line has been used in dark toxicity testing of a chloro-indium-phthalocyanine photosensitizer (In-Pc). It has also been used to study the potential mechanisms and domain requirements of the hHSJ1a (DnaJ heat shock protein family member B2) mediated reduction in superoxide dismutase 1 (SOD1) aggregation.

Cell Line Origin

Human Caucasian neuroblastoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 8,13
D5S818: 12
D7S820: 7,10
THO1: 7,10
TPOX: 8,11
vWA: 14,18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Established from a bone marrow metastasis from a 4-year-old caucasian female suffering with neuroblastoma. The cells have been used as a target cell in cell-mediated cytoxicity tests.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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