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Sigma

WEHI 3B

86013003

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse blood
growth mode   Suspension
karyotype   Not specified
morphology   Not specified
products   Interleukin 3 (IL-3)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Tumourigenicity studies

Cell Line Origin

Mouse BALB/c myelomonocyte

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 1-5x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

Derived from a BALB/c mouse which has undergone mineral oil injections intended to induce plasma cell tumour development. The original tumour WEHI-3b showed many characteristics of human myelomonocytic leukaemia. The cells produce high levels of IL-3.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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Technical Service:

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