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1321N1 Cell Line human

from human brain(astrocytoma), 86030402

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Properties

Related Categories 0-10, Cell Culture, ECACC Cell Lines More...
biological source   human brain (astrocytoma)
growth mode   Adherent
karyotype   Not specified
morphology   Glial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human brain astrocytoma

Cell Line Description

1321N1 is a human astrocytoma cell line isolated in 1972 as a sub clone of the cell line 1181N1 which in turn was isolated from the parent line U-118 MG (one of a number of cell lines derived from malignant gliomas by J Ponten .1321N1 has been shown to have very similar STR profile data to U-118 MG.

Application

Human 1321N1 Cell Line has been used to study how the multiscale morphology of functional thin films affects the in vitro behavior of human neural astrocytoma 1321N1 cells. It has also been used to study the expression and reactivity of Caytaxin protein.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 12,13
D5S818: 11
D7S820: 9
THO1: 6
TPOX: 8
vWA: 18

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

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