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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse embryo
growth mode   Adherent
karyotype   Not specified
morphology   Fibroblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse SIM embryo fibroblast

Cell Line Description

Selected for 6-thioguanine and ouabain resistance; sensitive to HAT; HPRT negative. Used routinely as feeder layers prepared by irradiation or mitomycinc treatment for maintenance of teratocarcinoma stem cells in the undifferentiated state.

Application

Oubain resistance, feeder layer

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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