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Sigma

V79

86041102

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   hamster lung
growth mode   Adherent
karyotype   2n=22
morphology   Fibroblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr D Wynford-Thomas, Department of Pathology, University of Wales, College of Medicine

Application

Growth factors and transformation studies, mutagenesis study

V79 has been used to detect structural and numerical chromosome damage by measuring the formation of micronuclei in interphase cells that have been through a mitotic division.

Cell Line Origin

Hamster Chinese lung male

Culture Medium

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:12 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Cell Line Description

Established after spontaneous transformation of cells isolated from the lung of a normal Chinese hamster (male). The G1 phase in these cells is either absent or very short. Consequently they have been used in studies of the G1 growth period. A clone called V79.4 is available (Sigma Catalogue number. 93010723). V79 is well established in toxicology studies. Stability of karyotype and morphology is making them suitable for gene toxicity assays with low background aberrations. The cells do not express endogenous cytochrome P450 and have been used for the insertion and expression of P450 from human, rat, mouse and fish.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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