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IM 9

86051302

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Properties

Related Categories Cell Culture, D-I, ECACC Cell Lines More...
biological source   human bone marrow
growth mode   Suspension
karyotype   2n = 46
morphology   Lymphoblast
products   Immunoglobulin G (IgG)
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human B lymphocyte

Cell Line Description

Derived from bone marrow removed from a female patient with multiple myeloma. The cells synthesise IgG at a rate of 2.4ug/1,000,000 cells/day and have receptors for insulin, calcitonin and human growth hormone. They also exhibit surface markers and receptor sites characteristic of B lymphocytes.

Application

Biosynthetic and surface marker studies

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 2-4x100,000 cells/ml; 5% CO2; 37°C. Maximum cell density 1,000,000 cells/ml.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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