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AFRC MAC 61

Reactivity: Anti-Abscisic acid, plant growth hormone, 86052301

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Properties

Related Categories Cell Culture, ECACC Cell Lines, Hybridoma Collection More...
biological source   Rat x Rat Hybridoma
ImmunoDon   F344 rat spleen
Myeloma   YB2/3.0 Ag20
Reactivity   Anti-Abscisic acid, plant growth hormone
application(s)   cell culture | mammalian: suitable
isotype   IgG2c
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Rat YB2/3.0 Ag20 x Rat F344 spleen

Cell Line Description

The antibody is directed against the plant growth hormone cis, trans-abscisic acid. The antibody production rate of this hybridoma has been tested at ECACC. It must be noted that this hybridoma produces less than 2μg/ml of unpurified antibody.

Immunogen

S-cis, trans-abscisic acid-4′-p-amino-benzoyl hydrazone-KLH conjugate

Culture Medium

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. These cells require mouse macrophage feeder layers upon resuscitation

Other Notes

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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