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HEL 299

87042207

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Properties

Related Categories Cell Culture, D-I, ECACC Cell Lines More...
biological source   human lung (embryonic)
growth mode   Adherent
karyotype   2n = 46, diploid
morphology   Fibroblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human embryo lung

Cell Line Description

Derived from embryonic lung tissue of a male in 1965. It is one of the few human diploid lines to have type A glucose-6-phosphate dehydrogenase. Cells have a limited lifespan of 20 serial subcultures from tissue of origin. Ethnicity: Black.

Application

HEL 299 has been used to study the antiviral properties of AIC246, a novel anti-human cytomegalovirus compound. It has also been used to study the role of SUMO ligase protein inhibitor of activated STAT1 (PIAS1) in the intrinsic antiviral immune response to herpes simplex virus.

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 7,10
D13S317: 11,12
D16S539: 10,11
D5S818: 11,13
D7S820: 8,11
THO1: 7
TPOX: 8,12
vWA: 16

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Limited lifespan.

Other Notes

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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