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Sigma

Namalwa

87060801

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Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   human blood
growth mode   Suspension
karyotype   Modal no. 44; hypodiploid
morphology   Lymphoblast
products   Immunoglobulin M (IgM), lambda
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Burkitt′s Lymphoma

Cell Line Description

Secretes small amounts of an IgM monoclonal antibody of unknown specificity. It has been used for commercial production of human interferon. The cells contain the EBV genome and should be handled under laboratory containment level 2.

Application

Interferon studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 11,12
D16S539: 9
D5S818: 12,13
D7S820: 11
THO1: 7,9.3
TPOX: 6,11
vWA: 14

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. A maximum density of 2x1,000,000 cells/ml is possible.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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Pipette Controller from Brand

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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