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HOS

87070202

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Properties

Related Categories Cell Culture, D-I, ECACC Cell Lines More...
biological source   human bone
growth mode   Adherent
karyotype   2n = 46, hyperdiploid
morphology   Epithelial-like
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Caucasian osteosarcoma, TE85

Cell Line Description

Derived from a 13 year old female caucasian. Cells are sensitive to both virus and chemical transformation and exhibit a flat morphology, low saturation density and low plating efficiency in soft agar. HOS and 143B (Sigma Catalogue number 91112502) originated from the same host: 143B is TK- and originated indirectly from HOS which is TK+ (Skovgaard et al 2010 PMID: 20544518).

Application

Virus, transformation and biochemical studies

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 12
D16S539: 10,13
D5S818: 13
D7S820: 11,12
THO1: 6
TPOX: 8,11
vWA: 18

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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