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Sigma

L1210

87092804

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Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   mouse blood
growth mode   Suspension
karyotype   2n=40, diploid
morphology   Lymphoblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cell Line Origin

Mouse DBA/2 lymphocytic leukaemia

Culture Medium

Fischer′s medium + 2mM Glutamine + 10% Horse Serum (HS).

Subculture Routine

Maintain cultures between 5x10,000 to 8x100,000 cells/ml; 5% CO2; 37°C.

Cell Line Description

Established from a tumour induced in an 8 month old female 234 subline 212 of a DBA mouse. Orginally subcultured in DBA/2 mice it was adapted to suspension culture. The line has been extensively used in cytotoxicity assays. Develops massive tumours in nude mice after a latent period of 7-10 days

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles
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