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Sigma

COLO 320HSR

87101501

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Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   human colon
growth mode   Suspension
karyotype   Hyperdiploid, modal no. 52
morphology   Round & Refractile
products   Serotonin, epinephrine, NPP, Adrenocorticotropic hormone (ACTH) and PTH
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Caucasian colon adenocarcinoma

Cell Line Description

Subclone of COLO320DM, characterised by marker chromosomes with homogeneously staining regions (HSR) and double minutes (DM) at very low levels.

Application

Hormone studies

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Will form clusters in suspension.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Prof G Moore, Colorado Oncology Foundation, Denver General Hospital, Co 80204-4507

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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