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Sigma

T84 Cell Line human

88021101

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human colon (lung metastasis)
description   Human colon carcinoma
growth mode   Adherent
karyotype   2n = 46, modal no. 56
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   metastasis
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human colon carcinoma

Cell Line Description

Derived from a lung metastasis of colon carcinoma in a 72 year old male. Tumour tissue was inoculated sub-cutaneously and serially transplanted in BALB/c nude mice and subsequently established in in vitro culture. The histological characteristics of the tumour were maintained throughout the transplantation procedure. They have receptors for a range of peptide hormones and neurotransmitters. The cells grow as monolayers, exhibit tight junctions and desmosomes between adjacent cells.

Application

Human T84 cell line has been used for comparative genome analysis of Salmonella enterica serovar Typhimurium definitive type 2 (DT2) isolate 94-213 and S. Typhimurium isolates SL1344, DT104 and D23580. The ability of these isolates to invade culture medium has also been studied. It has also been used to construct and study the suitability of an in vitro model to study chemotherapy-induced mucosal damage.

Tumourigenicity, neurotransmitter and hormone studies

Culture Medium

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 - 1:4 i.e. seeding at 1-3 x 10,000 cells / cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Cells grow slowly and may not form a complete monolayer, maintain at high density with a minimum of 25% confluency.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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