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Jurkat E6.1 Cell Line human

from human blood(leukemic T-cell lymphoblast), 88042803

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Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   human blood (leukemic T-cell lymphoblast)
growth mode   Suspension
karyotype   Pseudodiploid, modal No 46
morphology   Not specified
products   Interleukin 2 (IL-2), human alpha interferon
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human leukaemic T cell lymphoblast

Cell Line Description

Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.

Application

IL-2 assay and cloning studies

Jurkat E6.1 cell line has been used to:
• study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
• determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
• study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro

DNA Profile

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

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If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
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The lot specific COA document can be found by entering the lot number above under the "Documents" section.
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