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Sigma

TM4

88111401

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   mouse testis
growth mode   Adherent
karyotype   Not specified
morphology   Not specified
products   Transferrin, H-Y Ag, novel retinol-binding protein
receptors   Androgen, oestrogen and progesterone receptors
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mouse BALB/c testis sertoli cell

Cell Line Description

From primary culture of sertoli cell enriched preparations from normal testes of a 11-13 day old BALB/c nu/+ mice. Cells respond to FSH but not LH; Plasminogen activator secretion is stimulated by FSH and also retinoic acid. Note: only distributed frozen.

Application

Hormone studies

Culture Medium

Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:20 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cells resuscitated from frozen stock should be split 1:3 at the first passage and thereafter as above.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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