Related Categories Cell Culture, ECACC Cell Lines, O-R More...
biological source   rat testis
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
products   Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C


Cell Line Origin

Rat testis Leydig cell tumour

Cell Line Description

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

Culture Medium

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin/EDTA;5% CO2; 37°C.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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