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Clone C6/36

89051705

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Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   insect larvae
growth mode   Adherent
karyotype   Diploid
morphology   Not specified
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Mosquito larvae, Aedes albopictus

Cell Line Description

Derived from Aedes albopictus cells. Useful for propagation of Dengue virus and flaviviruses. Cells are non-anchorage dependant, not tumourigenic and maintain a diploid chromosome number.

Application

Clone C6/36 has been used to screen anti-Wolbachia compounds. It has also been used to study the effects of tick-borne encephalitis virus (TBEV) vaccine.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (Sodium Bicarbonate (NaHCO3) level of 1.1g/l is recommended).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 5000 - 30000 cells/cm2 . Cells are dislodged by mechanical agitation; 5% CO2; 28°C. Freeze in 10% glycerol.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles
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