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Sigma

AGS Cell Line human New

89090402

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Properties

Related Categories A-C, Cell Culture, ECACC Cell Lines More...
biological source   human stomach
description   Human Caucasian gastric adenocarcinoma
growth mode   Adherent
karyotype   Hyperdiploid
morphology   Not specified
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

AGS human cell line has been used:
• to study the role of obestatin/G protein-coupled receptor 39 (GPR39) in gastric cancer progression
• to determine expression levels of colon cancer associated transcript -1 (CCAT1)
• to study the adhesion of Lactobacillus sps. to the gastric mucosa

Anti-tumour testing

Cell Line Origin

Human Caucasian gastric adenocarcinoma

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 11,13
D5S818: 9,12
D7S820: 10,11
THO1: 6,7
TPOX: 11,12
vWA: 16,17

Culture Medium

Ham′s F12 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 0.05% EDTA; 5% CO2; 37°C.

Cell Line Description

Derived from an adenocarcinoma of the stomach of a 54 year-old Caucasian female with no prior anti-cancer treatment. AGS cells are persistently infected with parainfluenza virus type 5 (PIV5).

Safety & Documentation

Safety Information

Safety Information for this product is unavailable at this time.

Documents

Certificate of Analysis

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