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Sigma

WI 26 VA4

89101301

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human lung (fetal)
growth mode   Adherent
karyotype   2n = 46, unstable karyotype
morphology   Epithelial-like
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Transformation and virus studies: SV40

Cell Line Origin

Human Caucasian foetal lung, SV40 transformed

Culture Medium

DMEM + 10% Foetal Bovine Serum (FBS) + 2mM Glutamine.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Cell Line Description

An SV40 transformed derivative of WI-26, a human diploid cell line derived from embryonic lung tissue of a male Caucasian. The cells have SV40 T-Ag but infectious virus has not been rescued.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis


Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
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