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Sigma

WILCL

89120565

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Properties

Related Categories Cell Culture, ECACC Cell Lines, S-Z More...
biological source   human blood
growth mode   Aggregates in suspension
karyotype   Normal
morphology   Lymphoblast
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human Caucasian normal B cell

Cell Line Description

Normal B cell line of a patient with a lung tumour. LUDLU-23, a lung squamous cell carcinoma, is also derived from the same patient.

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Cells grow as large cell aggregates; resuscitate in 8ml media with flask standing up until cells start to proliferate well.

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Dr P Rabbitts, MRC, Clinical Oncology and Radiotherapeutics Unit, Cambridge

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Cell Culture Guide

ECACC Laboratory Handbook
Protocols & Articles
Related Products

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