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Neuro 2a Cell Line from mouse

89121404

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Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   mouse nerve
description   Mouse Albino neuroblastoma
growth mode   Adherent
karyotype   2n = 40
morphology   Neuronal/amoeboid-like
products   Microtubular protein
receptors   Not specified
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cancer
shipped in   dry ice
storage temp.   −196°C

Description

Other Notes

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Application

Neuro 2a cell line has been used as a model to study the potential toxicological effects of copper oxide nanoparticles accumulation in the brain. It has also been used to determine the sensitivity of the cell line to saxitoxin.

Tumourigenicity studies, microtubular protein synthesis, vinblastine precipitation, GTP binding kinetics

Cell Line Origin

Mouse Albino neuroblastoma

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 4x10,000 cells cm2; 5% CO2; 37°C. After resuscitation cells can take up to 6 days before a monolayer forms. These cells only adhere to the culture flask very lightly, care should be taken when subculturing. There is no need to wash the cells with PBS or trypsin, merely pour off half of the spent medium carefully, give the flask a gentle knock and resuspend the cells in fresh medium using a pipette to gently disperse any clumps of cells to finally reseed at 1:2. On arrival, growing cultures should be spun out and re-seeded in fresh media.

Cell Line Description

Derived from a spontaneous tumour in an albino strain A mouse. Cells produce microtubular protein which is believed to play a role in the contractile system giving axoplasmic flow in nerve cells.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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