EMAIL THIS PAGE TO A FRIEND

Nthy-ts1

90011610

Purchase

Properties

Related Categories Cell Culture, ECACC Cell Lines, J-N More...
biological source   human thyroid
growth mode   Adherent
karyotype   Not specified
morphology   Epithelial
products   Not specified
receptors   Not specified
application(s)   cell culture | mammalian: suitable
shipped in   dry ice
storage temp.   −196°C

Description

Cell Line Origin

Human thyroid follicular mutant epithelial

Cell Line Description

Normal primary cultures of human thyroid follicular cells were transfected with a plasmid containing the SV40 early region large T encoding gene from mutant tsA58. Tight temperature dependence for growth is observed: Nthy-ts1 cells grow at 33°C, but not at the restrictive temperature of 40.5°C. This SV40 mutant allows the study of oncogene action free of any interfering effects of SV40 large T antigen.

Application

Transfection, mutation and virus studies: SV40

Culture Medium

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 33°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles
Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?